Analytical methods for the quality control of pharmaceutical raw materials and finished products are routinely transferred between laboratories, including contract research organizations and competent authority/official medicines control laboratories (OMCLs) equipped with diverse instrumentation. Successful method transfer is essential to ensure reproducible results, regulatory compliance, and consistent product quality, while minimizing the need for partial or full method re-validation. Reversed-phase high-performance liquid chromatography (RP-HPLC) remains the dominant separation technique, accounting for more than 90% of chromatographic applications..........

Keywords : RP-HPLC; Method transfer; Related substances; Acetylsalicylic acid; Column selection.

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Background: Diabetic patients with high risk of end-stage renal failure due to albuminuria and death; ACEIs and ARBs are commonly used however comparative benefits remain unknown. Methods: 100 adults (≥18 years) with T2DM (Hb1c ≥6.5%),and with hypertension initiating ACEI (e.g., enalapril) or ARB (e.g., losaran) monotherapy (eGFR 30-90 mL/min/1.73m2), exclusions: prior RAS use, pregnancy, hyperkalemia. Baseline data (BP,eGFR, ACR, HbA1c) were analysed in the present prospective study (from clinic records; 24-month follow-up quarterly)...........

Keywords : ACEIs, ARBs, type 2 diabetes, hypertension, renal outcomes, blood pressure, albuminuria.

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N-octyl cinnamic is a compound that has potential as a drug as an antioxidant, anti-inflammatory, but is constrained by the nature of n-octyl cinnamate which is difficult to dissolve in water, causing low bioavailability in the systemic circulation. To overcome this problem, curcumin is formulated in the form of nanoparticles using chitosan polymer, a derivative of chitin which can be obtained from crab and shrimp shell waste. This study aims to synthesize n-octyl cinnamic nanoparticles using a simple coating method using chitosan and sodium alginate and to evaluate the stability of the nanoparticles in vitro............

Keywords : Nanoparticle, Anti-inflammatory, n-octyl cyanamate/Alginate/chitosan, Synthesis.

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Ipomoea hederifolia (Convolvulaceae family) haveshown long history of medicinal uses including folk remedies for many biological conditions. The current study's objectiveis to extract, isolate and characterize bioactivechemicals from the stem parts of I.hederifolia and alsomeasures the invitro antioxidant activity (using DPPH assay), antibacterial activity, total flavonoid and phenol content. Chemical examination of stem portion yieldedβ-sitosterol-3-O-β-D-glucosidecompound whose structure established on the bases of various spectral NMR data. Stempowder showed better Antioxidant activity in 30% aqeous methanol extract, more phenolic content in acetone extract and high flavonoidcontent in methanol extract(using Catechin standard)and acetone extract(using Quercetin standard), less antibacterial activity in invitro mode.

Keywords : Ipomoea hederifolia; Ascorbic acid;Gallic acid; DPPH; Quercetin; Catechin.

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Drying is the most important process to maintain the stability of chemical compounds from plants. Chemical compounds from plants that have medicinal properties, one of them is flavonoids which are secondary metabolites. To get high levels of flavonoids, we must keep to how to drain any plants. The aim of this study was to investigate the best drying methods wich obtained higher levels of flavonoids. The study was such an experimental analytical research consisting of two groups. The herbs was dried by two different methods aerated and sun heating. Extraction was conducted by maceration with 70% ethanol solvent. Flavonoid levels were calculated using UV-Vis spectrofometry. The average flavonoid content in the aerated group and the sun heating group 2 mg/gram and 1.89 mg/gram respectively.

Keywords : drying methods, flavonoids, patikankebo

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